Cell Therapy and Applied Genomics

The Department of Cell Therapy and Applied Genomics (CTAG) seeks to deliver and enable personalized medicine through cellular therapeutic and genomics based technologies to Jordan and its neighbors.  The CTAG department is currently working on establishing a national cord blood bank to serve patients who need a bone marrow transplant but do not have related donors.
 
Department Objectives:
  • Establish the first public/hybrid cord blood bank in the country
  • Establish a national stem cell donor registry
  • Introduce SNP microarrays for virtual karyotyping of cancer.
  • Introduce Luminex (xMAP) technology for HLA typing and other molecular and immunological applications.
 Our expertise
  1. Stem cell processing 
  2. Cord blood banking
  3. Transplant Immunology and Histocompatibility
  4. Flow cytometry
  5. Molecular Genetics Pathology
  6. Cytogentics and Molecular Cytogenetics
Cell Therapy and Bone Marrow Transplant (BMT) 
  • The Cell Therapy and Applied Genomics Department boasts a comprehensive BMT laboratory capable of supporting a fully-fledged BMT program
  • BMT patients can get all transplant-related work done at one site. The BMT lab at KHCC provides everything from collections, processing, cryopreservation and storage, to thawing of frozen cells for infusion from the same location
  • The BMT laboratory offers both autologous and allogeneic  donor protocols (related and unrelated) through a range of procedures from cell washing, plasma and/or red cell depletion, CD34+ cell selection and T-cell depletion by two different methods. Patients have the option to get autologous, related or unrelated blood/marrow transplant.
  • We accommodate both adult and pediatric patients
  • The BMT lab specializes in cell collection from very low-weight pediatric patients (9kg or more). It is the only lab in Jordan that can perform cell collections on such small patients
  • The BMT lab processes bone marrow, peripheral blood derived and cord blood derived hematopoietic progenitor cells. This way, physicians can evaluate the patient’s need and select the best stem cell source. It increases transplant eligibility of those patients who do not have related donors
  • The number of BMT transplants performed at KHCC is the highest in the country. This allows our staff to gain valuable expertise in BMT lab practice
  • The BMT lab provides Therapeutic Leukapheresis Granulocyte collection. Very few hospitals offer this critical care service
  • Detection of early onset of CMV through CMV antigenemia assay is offered by the BMT lab at KHCC. Around 100 of these procedures are performed every month.
Molecular Genetics and Immunogenetics
The Molecular Diagnostics and Immunogenetics (MDI) Laboratory at KHCC is a College of American Pathologists (CAP)-certified clinical diagnostic facility specializing in oncologic and infectious molecular testing
  • We provide a roster of testing for leukemia, lymphoma and a variety of other malignancies. Our laboratory can analyze samples from patients at King Hussein Cancer Center as well as serve as a referral laboratory for other hospitals and clinics.
  • We are dedicated to providing patients and physicians with comprehensive and accurate molecular testing. The lab utilizes the strength of molecular techniques such as PCR, qPCR, Genetic Sequencing, RFLP and Gene Mapping for diagnostic and research purposes when needed. We provide a wide range of DNA and RNA based tests for inherited genetic diseases, hematologic malignancies and infectious diseases all of which are performed at King Hussein Cancer Center.
  • One of our primary strengths is molecular testing of leukemia. In this regard, we work closely with other KHCC Pathology laboratories including the Clinical Cytogenetic, Flow Cytometry, and Immunohistochemistry laboratories to provide an integrated approach to detecting disease-specific diagnostic and prognostic molecular markers. Our lab also performs DNA-based human leukocyte antigen (HLA) typing and chimerism analysis services for the Stem Cell Transplant Program at KHCC
Flow Cytometry
  • The Flow Cytometry lab contributes an important part to clinical diagnosis of hematopathological diseases, lymphomas, BMT transplantation patients, minimal residual disease detection, Paroxysmal Nocturnal Hemoglobinuria and immunodeficiency diseases.
  • The only Flow Cytometry lab accredited by CAP in the country.
  • Protocols and QC are followed daily to monitor the performance of an analytical system to ensure the reliability of the process or measurement performed on a patient sample. An effective QC program identifies areas for process improvement and ensures reliable results for both short-term and long-term medical decisions.
  • The Flow Cytometry laboratory is enrolled in the College of American Pathologists (CAP) Survey for Paroxysmal nocturnal hemoglobinuria (PNH), Leucocyte Immunophenoyping for leukemia and Lymphoma testing subset enumeration and CD34 stem cell quantitation and Transfusion Related Cell Count (TRC).
Department Faculty
Dr. Abdelghani Tbakhi - Chairman 
 
Alphabetical KHCC Laboratories Tests List and Collection Requirements:
King Hussein Cancer Center provides all concerned parties with a directory of tests needed for cancer patients. This directory contains test name, requirements of collection, performance information, Turn out time, and special handling information.
 
For detailed information about the Alphabetical KHCC Laboratories Tests List and Collection Requirements please click here